Journal: Cancer Genomics & Proteomics

Article Title: Targeting Bmi1 for Enhancing Anoikis Sensitivity and Inhibiting Metastasis in Colorectal Cancer

doi: 10.21873/cgp.20469

Figure Lengend Snippet: The level of Bmi1 expression dictates the metastatic potential of colorectal cancer (CRC) cells. (A) Formalin-fixed paraffin-embedded samples from four pairs of CRC patients were stained with the anti-Bmi1 antibody (magnification, 200×). (B) Bmi1 expression was evaluated via western blotting using anti-Bmi1 antibodies in SW480 and SW620 cells. (C) Migration of SW620 cells and Bmi1-depleted cells in zebrafish. (D) Expression of Bmi1 was analyzed via western blotting using anti-Bmi1 antibodies in suspended SW620 cells at specified time points.

Article Snippet: A human colorectal cancer tissue microarray (CDA3, Biomax Inc. and Super Bio Chips Laboratories, Seoul, Republic of Korea) was utilized.

Techniques: Expressing, Formalin-fixed Paraffin-Embedded, Staining, Western Blot, Migration

Journal: Cancer Genomics & Proteomics

Article Title: Targeting Bmi1 for Enhancing Anoikis Sensitivity and Inhibiting Metastasis in Colorectal Cancer

doi: 10.21873/cgp.20469

Figure Lengend Snippet: Bmi1 regulates anoikis resistance in colorectal cancer (CRC) cells. (A) Migration of SW620 cells and Bmi1-depleted cells was assessed using a Transwell assay after 24 h of growth in suspension. (B) The percentage of apoptotic SW620 cells and Bmi1-depleted cells at specified time points in suspension was determined by dual staining with annexin V-FITC and PI. The experiment was replicated thrice, and between-group comparisons were performed using a one-way ANOVA (*p<0.05). (C) The percentage of apoptotic SW620 cells treated with the Bmi1 inhibitor PTC209 at specified time points in suspension was determined by dual staining with annexin V-FITC and PI. The experiments were replicated thrice, and between-group comparisons were performed using a one-way ANOVA (*p<0.05). (D) Expression levels of Bmi1 and cleaved caspase-3 were assessed via western blotting after 48 h of suspension.

Article Snippet: A human colorectal cancer tissue microarray (CDA3, Biomax Inc. and Super Bio Chips Laboratories, Seoul, Republic of Korea) was utilized.

Techniques: Migration, Transwell Assay, Suspension, Staining, Expressing, Western Blot

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration

doi: 10.1155/2018/8623937

Figure Lengend Snippet: CapG expresses in the human colorectal carcinoma and normal specimens in tissue microarray. A tissue microarray was used to examine the expression of CapG by immunohistochemistry; the expression index of CapG in the human colon tissues was quantified by a pathologist, and scores of the specimens were also organized depending on the pathologic diagnosis with normal, colorectal carcinoma, and metastatic colorectal carcinoma. ∗ p < 0.05.

Article Snippet: A human colorectal cancer-metastasis-normal tissue microarray slide (CDA3) was purchased from SuperBioChips Laboratories (Seoul, Korea).

Techniques: Microarray, Expressing, Immunohistochemistry, Biomarker Discovery

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration

doi: 10.1155/2018/8623937

Figure Lengend Snippet: The mRNA and protein expression levels of CapG in four human colorectal carcinoma cell lines. Four human CRC cell lines including HT29, DLD-1, HCT116, and SW1116 were used to analyze the expressions of (a) mRNA and (b) protein of CapG. GAPDH was used as a loading control.

Article Snippet: A human colorectal cancer-metastasis-normal tissue microarray slide (CDA3) was purchased from SuperBioChips Laboratories (Seoul, Korea).

Techniques: Expressing, Control

Journal: Clinical Science (London, England : 1979)

Article Title: ANGPTL1 attenuates cancer migration, invasion, and stemness through regulating FOXO3a-mediated SOX2 expression in colorectal cancer

doi: 10.1042/CS20220043

Figure Lengend Snippet: ( A ) The expression of ANGPTL1 negatively correlates with CRC, metastatic stage, and recurrence. ONCOMINE datasets: Hong_Colon, Bitner_Colon, and Jorissen_Colon. ( B ) IHC staining of ANGPTL1 in colon and CRC TMA tissues with scores of 0–3 was represented. Positive control: Carbon; scale bar: 100 μm. ( C ) The box plot representation of scores based on IHC staining of ANGPTL1 in 59 paired specimens of CRC and adjacent normal colon tissues. P -value was analyzed by Chi-square analysis followed by Fisher’s exact test. ( D ) The overall survival of 58 patients in TMA with low and high ANGPTL1 expression was analyzed by Kaplan–Meier analysis ( P =0.0372). ANGPTL1 expression was grouped based on the median of the IHC score of specimens. ( E ) ANGPTL1 expression inversely associated with metastatic tissues compared with paired specimens of CRC tissues in TMA ( n =10). * P <0.05 was obtained by paired two-tailed Student’s t -tests.

Article Snippet: CRC human tissue microarrays (TMA) were purchased form SuperBioChips (Seoul, Korea), including 59 paired normal colon and rectum tissues (CDN4) and tumor tissues (CD4), and human CRC metastasis-normal tissues (CDA3).

Techniques: Expressing, Immunohistochemistry, Positive Control, Two Tailed Test

Journal: Cells

Article Title: Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins

doi: 10.3390/cells7120262

Figure Lengend Snippet: Primer sequences used QPCR analysis with gene name.

Article Snippet: For determination of the association of HSP27 and STIM1 expression levels, two consecutive formalin-fixed paraffin-embedded tissue microarray sections purchased from SuperBioChips Laboratories (catalog no. CDA3, Seoul, Korea) were stained with HSP27 and STIM1 (catalog no. HPA012123, 1:200, Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden) by immunohistochemistry using the aforementioned protocol.

Techniques: Sequencing

Journal: Cells

Article Title: Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins

doi: 10.3390/cells7120262

Figure Lengend Snippet: HSP27 increased the STIM1 protein levels in CRC cells. ( a ) QPCR was used to determine the STIM1 mRNA levels, and the levels were normalized to the GAPDH level. ( b , c and d ) Protein extracts prepared from the indicated cell lines were subjected to western blot analysis for STIM1 or Orai1. GAPDH was used as an internal control. Data are representative of three independent experiments. Values are the mean ± SD of three independent experiments. ** p < 0.01.

Article Snippet: For determination of the association of HSP27 and STIM1 expression levels, two consecutive formalin-fixed paraffin-embedded tissue microarray sections purchased from SuperBioChips Laboratories (catalog no. CDA3, Seoul, Korea) were stained with HSP27 and STIM1 (catalog no. HPA012123, 1:200, Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden) by immunohistochemistry using the aforementioned protocol.

Techniques: Western Blot, Control

Journal: Cells

Article Title: Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins

doi: 10.3390/cells7120262

Figure Lengend Snippet: HSP27 prevented STIM1 degradation by directly binding to STIM1. ( a , b ) Scrambled control or HSP27KD cells were treated with cycloheximide (CHX), MG132 or PYR-41. Whole cell lysates were immunoblotted for STIM1 and HSP27. GAPDH was used as an internal control. ( c ) The possibility of direct protein-protein interactions was examined by immunoprecipitation (IP). All the experiments were independently performed at least 3 times.

Article Snippet: For determination of the association of HSP27 and STIM1 expression levels, two consecutive formalin-fixed paraffin-embedded tissue microarray sections purchased from SuperBioChips Laboratories (catalog no. CDA3, Seoul, Korea) were stained with HSP27 and STIM1 (catalog no. HPA012123, 1:200, Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden) by immunohistochemistry using the aforementioned protocol.

Techniques: Binding Assay, Control, Protein-Protein interactions, Immunoprecipitation

Journal: Cells

Article Title: Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins

doi: 10.3390/cells7120262

Figure Lengend Snippet: HSP27 promoted STIM1 oligomerization in DLD-1 cells. Scrambled control or HSP27KD DLD cells were seeded into coverslips and then treated with vehicle or TG for 15 min. Cells were fixed, and the distribution of STIM1 was detected by immunocytochemical staining. Confocal microscopy was applied to observe the distribution of STIM1 (green). Nuclei were identified by DAPI. All experiments were independently performed at least 3 times.

Article Snippet: For determination of the association of HSP27 and STIM1 expression levels, two consecutive formalin-fixed paraffin-embedded tissue microarray sections purchased from SuperBioChips Laboratories (catalog no. CDA3, Seoul, Korea) were stained with HSP27 and STIM1 (catalog no. HPA012123, 1:200, Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden) by immunohistochemistry using the aforementioned protocol.

Techniques: Control, Staining, Confocal Microscopy

Journal: Cells

Article Title: Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins

doi: 10.3390/cells7120262

Figure Lengend Snippet: The association between HSP27 and STIM1 expression. ( a ) The scatterplot of H-scores of HSP27 and the corresponding STIM1 expression displayed a positive correlation (correlation coefficient = 0.416, p = 0.003). ( b ) A case of colon adenocarcinoma with a high HSP27 H-score displayed a high STIM1 H-score. ( c ) A case of colon adenocarcinoma with a low HSP27 H-score displayed a low STIM1 H-score.

Article Snippet: For determination of the association of HSP27 and STIM1 expression levels, two consecutive formalin-fixed paraffin-embedded tissue microarray sections purchased from SuperBioChips Laboratories (catalog no. CDA3, Seoul, Korea) were stained with HSP27 and STIM1 (catalog no. HPA012123, 1:200, Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden) by immunohistochemistry using the aforementioned protocol.

Techniques: Expressing

Journal: Cells

Article Title: Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins

doi: 10.3390/cells7120262

Figure Lengend Snippet: The proposed model for the role of HSP27 in CRC progression. HSP27 may interact with STIM1 to maintain the stability of STIM1. Silencing HSP27 caused a reduction of STIM1 to regulate the growth activity and metastasis ability on CRC.

Article Snippet: For determination of the association of HSP27 and STIM1 expression levels, two consecutive formalin-fixed paraffin-embedded tissue microarray sections purchased from SuperBioChips Laboratories (catalog no. CDA3, Seoul, Korea) were stained with HSP27 and STIM1 (catalog no. HPA012123, 1:200, Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden) by immunohistochemistry using the aforementioned protocol.

Techniques: Activity Assay